High-performance liquid chromatographic determination of lombricine and N-phosphoryl lombricine in the earthworm by pre-column fluorescence derivatisation with o-phthaldialdehyde-ethanethiol

✍ Scribed by Melvin R. Euerby; Lynda Z. Partridge; William A. Gibbons

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 454 KB
• Volume: 445
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)84558-1

✦ Synopsis

Lombricine (2-guanidinoethyl-2-amino-2-carboxyethyl hydrogen phosphate) and its N-phosphorylated form (Fig. 1) have been reported to be present in a number of oligochaetes'+ including many species of earthworm. The physiological role of these compounds has been postulated lp3q7 to be similar to that of creatine and arginine and their corresponding N-phosphorylated forms in vertebrates and invertebrates respectively, in that they act as intracellular regulators of adenosine triphosphate levels. NH 0 NH,