High performance liquid chromatographic determination of 1,5-anhydroglucitol in human plasma for diagnosis of diabetes mellitus

✍ Scribed by Shigeo Tanaka; Kota Nakamori; Hiroshi Akanuma; Masahiko Yabuuchi

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 335 KB
• Volume: 6
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1130060204

✦ Synopsis

Abstract

This paper describes a high performance liquid chromatographic (HPLC) method for determining 1,5‐anhydroglucitol in plasma, in which anion exchange chromatography and pulsed amperometric detection are used. Plasma samples deproteinized with trichloroacetic acid are passed through a three‐layer column packed with (1) strongly basic anion (BO form, the upper layer), (2) strongly basic anion (OH^−^ form, the middle layer) and (3) strongly acidic cation (H^+^ form, the lower layer) exchange resins. 1,5‐Anhydroglucitol is efficiently recovered in the flow‐through fraction and interfering substances are completely removed by the column treatment. The analytical response of the method is linear with concentration to 40 mg/L, and it is possible to detect as little as 0.1 mg 1,5‐anhydroglucitol per litre of plasma. Analytical recovery is between 96 and 103%, and there is good agreement between the results measured by our method and by a gas/liquid chromatographic method (r = 0.998). The method has been successfully used for the determination of very low 1,5‐anhydroglucitol concentrations (< 1 mg/L) in the plasma of diabetic patients.