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High-performance liquid chromatographic assay for amiodarone N-deethylation activity in human liver microsomes using solid-phase extraction

โœ Scribed by Nobumitsu Hanioka; Yoshiro Saito; Akiko Soyama; Masanori Ando; Shogo Ozawa; Jun-ichi Sawada

Book ID
108497307
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
161 KB
Volume
774
Category
Article
ISSN
1873-376X

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High-Performance Liquid Chromatographic
High-Performance Liquid Chromatographic Assay for N-Glucuronidation of Nicotine and Cotinine in Human Liver Microsomes
โœ Miki Nakajima; Jun-Tack Kwon; Eriko Tanaka; Tsuyoshi Yokoi ๐Ÿ“‚ Article ๐Ÿ“… 2002 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 79 KB

A method for the determination of N-glucuronidation of nicotine and cotinine in human liver microsomes by high-performance liquid chromatography was developed. Nicotine or cotinine was incubated with human liver microsomes and UDP-glucuronic acid in a 200-l incubation mixture. The nicotine Nglucuron