High-performance liquid chromatographic analysis of major carotenoids from Rhodotorula glutinis

✍ Scribed by H.S. Nam; S.Y. Cho; J.S. Rhee

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 166 KB
• Volume: 448
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)84610-0

✦ Synopsis

A potent intracellular lipid-producing yeast, Rhodotorula glutinis, produces carotenoid pigments mainly during the stationary phase of its growth and the characteristics of the carotenoid pigments have been well established and four major carotenoids, i.e., torulene, torularhodin, B-carotene and y-carotene were identified1-3.

Until recently, column chromatography has been mainly used for the microbial carotenoids including this organism 2 ' However, this technique has its limitations -. such as the large quantity of sample required, poor resolution, incomplete recovery and artifact production 6. On the other hand, the use of high-performance liquid chromatography (HPLC) offers several advantages over classical chromatography including high effrency, selectivity, speed and mild conditions'.

Although both plant carotenoids*-l2 and plasma carotenoids'"r5 were analyzed by HPLC, there are few reports on the HPLC analysis of microbial carotenoids except for a few examples l6 Moreover, to our knowledge, no published methods can .