High-performance liquid chromatographic analysis of DNA composition and DNA modification by chloroacetaldehyde
โ Scribed by Ram P. Singhal; J.Patrick Landes
- Book ID
- 108335236
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 884 KB
- Volume
- 458
- Category
- Article
- ISSN
- 1873-3778
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๐ SIMILAR VOLUMES
A significantly improved method for base composition analysis of synthetic oligodeoxyribonucleotides is presented. This highly accurate and sensitive method uses enzymatic digestion followed by high-resolution HPLC of the nucleosides to determine the empirical base composition of the parent compound
DNA base composition was determined by reversed-phase high-performance liquid chromatography (HPLC). DNA was hydrolysed into nucleosides with nuclease P1 and bacterial alkaline phosphatase. The mixture of nucleosides was applied to HPLC without any further purification. One determination by chromato