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High-performance gas Chromatographie method for the estimation of the indole-3-acetic acid content of plant materials

✍ Scribed by William J. Hunter


Book ID
108336578
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
364 KB
Volume
362
Category
Article
ISSN
1873-3778

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πŸ“œ SIMILAR VOLUMES


The separation of indole-3-acetic acid a
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Eleven indole derivatives of biological significance were added to an extract of etiolated pea seedlings and the mixture was separated on a column of Sephadex G-15, by the following sequence of eluants: ethylenediamine-acetate, pH 4.2, ethylenediamine-acetate, pH 7.4, distilled water, and a methanol

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The use of high-performance liquid chromatography in the reversed-phase mode coupled to a spectrophotofluorimetric detector allowed the selective detection of acidic indoles possessing a C-3 methylene side chain, following the reaction of a purified plant extract with acetic anhydride. Of these acid