High-performance gas Chromatographie method for the estimation of the indole-3-acetic acid content of plant materials
β Scribed by William J. Hunter
- Book ID
- 108336578
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 364 KB
- Volume
- 362
- Category
- Article
- ISSN
- 1873-3778
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π SIMILAR VOLUMES
Eleven indole derivatives of biological significance were added to an extract of etiolated pea seedlings and the mixture was separated on a column of Sephadex G-15, by the following sequence of eluants: ethylenediamine-acetate, pH 4.2, ethylenediamine-acetate, pH 7.4, distilled water, and a methanol
The use of high-performance liquid chromatography in the reversed-phase mode coupled to a spectrophotofluorimetric detector allowed the selective detection of acidic indoles possessing a C-3 methylene side chain, following the reaction of a purified plant extract with acetic anhydride. Of these acid