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High-Molecular-Weight DNA Polymerases from Mouse Myeloma : Purification and Properties of Three Enzymes

✍ Scribed by Henning J. HACHMANN; Axel G. LEZIUS

Book ID
115115030
Publisher
John Wiley and Sons
Year
1975
Tongue
English
Weight
681 KB
Volume
50
Category
Article
ISSN
1432-1327

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A facile two-step procedure was employed for simultaneous purification of glucose-6-phosphate dehydrogenase and malic enzyme from mouse (strain DBAIZJ) and Drosophila melanogaster. This involved the use of an 8-(6-aminohexyl)-amino-2',5'-ADP-Sepharsoe affinity column chromatography followed by DEAE