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Purification and molecular weight determination of glucose-6-phosphate dehydrogenase and malic enzyme from mouse and Drosophila

✍ Scribed by Chi-Yu Lee; Charles H. Langley; James Burkhart

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 564 KB
Volume: 86
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90797-2

No coin nor oath required. For personal study only.

✦ Synopsis

A facile two-step procedure was employed for simultaneous purification of glucose-6-phosphate dehydrogenase and malic enzyme from mouse (strain DBAIZJ) and Drosophila melanogaster.

This involved the use of an 8-(6-aminohexyl)-amino-2',5'-ADP-Sepharsoe affinity column chromatography followed by DEAE-Sephadex chromatography. The native and subunit molecular weights of these two homogeneous enzymes were determined by gel-filtration chromatography and SDS-polyacrylamide gel electrophoresis. From this study, it was concluded that the two enzymes are tetrameric and have native molecular weights between 200,000 and 280,000 in both species.

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