𝔖 Bobbio Scriptorium
✦   LIBER   ✦

High-level soluble expression of hIGF-1 fusion protein in recombinant Escherichia coli

✍ Scribed by Danping Zhang; Peilian Wei; Limei Fan; Jiazhang Lian; Lei Huang; Jin Cai; Zhinan Xu

Book ID
108254773
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
460 KB
Volume
45
Category
Article
ISSN
1873-3298

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

New fusion protein systems designed to g
New fusion protein systems designed to give soluble expression in Escherichia coli
✍ Gregory D. Davis; Claude Elisee; Denton M. Newham; Roger G. Harrison πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 214 KB

Three native E. coli proteins-NusA, GrpE, and bacterioferritin (BFR)-were studied in fusion proteins expressed in E. coli for their ability to confer solubility on a target insoluble protein at the C-terminus of the fusion protein. These three proteins were chosen based on their favorable cytoplasmi

Recombinant protein expression and solub
Recombinant protein expression and solubility screening in Escherichia coli: a comparative study
✍ Berrow, Nick S. ;BΓΌssow, K. ;Coutard, B. ;Diprose, J. ;Ekberg, M. ;Folkers, G. E πŸ“‚ Article πŸ“… 2006 πŸ› International Union of Crystallography 🌐 English βš– 522 KB

Producing soluble proteins in __Escherichia coli__ is still a major bottleneck for structural proteomics. Therefore, screening for soluble expression on a small scale is an attractive way of identifying constructs that are likely to be amenable to structural analysis. A variety of expression-screeni

Proteome-based identification of fusion
Proteome-based identification of fusion partner for high-level extracellular production of recombinant proteins in Escherichia coli
✍ Zhi-Gang Qian; Xiao-Xia Xia; Jong Hyun Choi; Sang Yup Lee πŸ“‚ Article πŸ“… 2008 πŸ› John Wiley and Sons 🌐 English βš– 689 KB

## Abstract Extracellular production of recombinant proteins in __Escherichia coli__ has several advantages over cytoplasmic or periplasmic production. However, nonpathogenic laboratory strains of __E. coli__ generally excrete only trace amounts of proteins into the culture medium under normal grow