A genetic strategy to enhance recombinant protein production is discussed. A small DNA bending protein, Fis, which has been shown to activate rRNA synthesis upon a nutrient upshift, was overexpressed in E. coli strain W3110 carrying vector pUCR1. Overexpres- sion of Fis during exponential growth was
Recombinant expression of selectively sulfated proteins in Escherichia coli
β Scribed by Liu, Chang C; Schultz, Peter G
- Book ID
- 109909698
- Publisher
- Nature Publishing Group
- Year
- 2006
- Tongue
- English
- Weight
- 196 KB
- Volume
- 24
- Category
- Article
- ISSN
- 1087-0156
- DOI
- 10.1038/nbt1254
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