High level secretion of single-chain antibody in Pichia expression system
β Scribed by D. Luo; N. Mah; M. Krantz; D. Wishart; F. Jacobs; Luis Martin
- Book ID
- 110370009
- Publisher
- Springer-Verlag
- Year
- 1997
- Tongue
- English
- Weight
- 366 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0951-208X
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## Abstract A continuous fermentation process has been developed in __Pichia pastoris (P. pastoris__) with the glyceraldehydeβ3βphosphate dehydrogenase (__GAP__) promoter in order to produce large quantities of recombinant human chitinase (rhβchitinase) for preclinical studies as a potential highβd
The Pichia pastoris system has the potential for very high level production of foreign proteins. This, together with the recent availability of this system in kit form, has changed Pichia from being an expression system with only specialized biotechnological applications to one that is widely used f