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High-level production and purification of a fully active recombinant dextransucrase from Leuconostoc mesenteroides NRRL B-512F

✍ Scribed by Claire Moulis; Audrey Arcache; Pierre-Claude Escalier; Marguerite Rinaudo; Pierre Monsan; Magali Remaud-Simeon; Gabrielle Potocki-Veronese


Book ID
109329403
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
288 KB
Volume
261
Category
Article
ISSN
0378-1097

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The production of dextransucrase, dextran and fructose by sucrose fermentation using Leuconostoc mesenteroides NRRL-B512(F) was studied in batch operation in a bioreactor with total working volume of 1.5 dm 3 . The effect of temperature (20 to 40 β€’ C), pH (5.5 and 6.7) and sucrose concentration (10

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Dextransucrase production from Leuconostoc mesenteroides NRRL B-5 12F was carried out in batch cultures under different conditions in static culture conditions. Small changes in the temperature had a significant effect on the enzyme production. A temperature of 23 "C gave the maximum yield of the en