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High Level Expression of Proopiomelanocortin in E. coli Cells using Optimized Codons

โœ Scribed by Konogami, Tadafumi; Watanabe, Kenji; Shimamoto, Shigeru; Basak, Ajoy; Yamaguchi, Hiroshi; Hidaka, Yuji


Book ID
119209537
Publisher
Biophysical Society
Year
2012
Tongue
English
Weight
42 KB
Volume
102
Category
Article
ISSN
0006-3495

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High-level soluble expression of isopeni
โœ J.E. Baldwin; J.M. Blackburn; J.D. Sutherland; M.C. Wright ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Elsevier Science ๐ŸŒ French โš– 890 KB

The Isopenicillin N Synthase genes from Cephalosporium acremonium, Penicillium chrysogenum and Aspergillus nidulans have been expressed at high levels in Escherichia coli under control of the trc or lac promoters. The enzymes are produced in soluble form in contrast to previously reported expression