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High-level expression and simple purification of recombinant human insulin-like growth factor I

โœ Scribed by Sun-ok Kim; Young Ik Lee


Book ID
119132384
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
746 KB
Volume
48
Category
Article
ISSN
0168-1656

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A human granulocyte colony-stimulating factor (hG-CSF) gene was syntheeized and inserted into a trp expression vector for overexpression in E. cm?. A strong expression vector was constructed, and a simple purification procedure including in vitro refolding was estabiliehed. The final productivity of