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High-frequency transformation ofLactobacillus caseiwith plasmid pHY300PLK by electroporation

✍ Scribed by Kenji Watanabe; Mitsuhiro Hamasaki; Yukihiko Nakashima; Yukari Kakita; Fumio Miake


Publisher
Springer
Year
1994
Tongue
English
Weight
697 KB
Volume
29
Category
Article
ISSN
0343-8651

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High frequency transformation of Bacillu
✍ Chang, Shing ;Cohen, Stanley N. πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 449 KB

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) tra