High-frequency shoot regeneration from leaves of the apple rootstock M26 (Malus pumilaMill.)

✍ Scribed by S. Predieri; F. Fasolo Fabbri Malavasi

• Book ID: 104622479
• Publisher: Springer
• Year: 1989
• Tongue: English
• Weight: 674 KB
• Volume: 17
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00046858

✦ Synopsis

Regeneration of shoots was achieved from in vitro leaves of M26 at frequencies close to 100% on a medium based on MS salts and LS vitamins, containing 4.4#M BA and 0.5~M NAA. Dark and red light gave the best results in inducing shoot regeneration. White light at high intensity helped development of regenerated shoots. Inorganic nitrogen could be reduced by 75% without negative effect, and the presence of NH~ was necessary for regeneration. Leaves were able to regenerate after a 3 kR irradiation (gamma rays), not after 4 kR. Optimal dose should be between 1 and 2 kR.