High frequency adventitious shoot induction and plant regeneration from leaves of statice
β Scribed by J.H. Jeong; H.N. Murthy; K.Y. Paek
- Book ID
- 110294528
- Publisher
- Springer
- Year
- 2001
- Tongue
- English
- Weight
- 210 KB
- Volume
- 65
- Category
- Article
- ISSN
- 0167-6857
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A procedure has been developed which allows high frequency adventitious shoot regeneration from immature cotyledons of pea. Prolific shoot regeneration occurred following an initial callus growth on a Murashige and Skoog (MS) medium containing 0.5 mg/l 6-benzylaminopurine (BAP) and 4 mg/l Ξ±-naphthal
Adventitious shoots developed from in vitro-grown leaves of Vitis vinifera cultivars Cabernet Sauvignon, French Colombard, Grenache, Thompson Seedless (syn. Sultana) and White Riesling, V. rupestris cv. St. George (syn. du Lot) and V. vinifera Γ rupestris cv. Ganzin 1. Leaf explants less than 15 mm
Regeneration of shoots was achieved from in vitro leaves of M26 at frequencies close to 100% on a medium based on MS salts and LS vitamins, containing 4.4#M BA and 0.5~M NAA. Dark and red light gave the best results in inducing shoot regeneration. White light at high intensity helped development of