๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

High frequency of genetic instability of microsatellites in human prostatic adenocarcinoma

โœ Scribed by Rajvir Dahiya; Celeste Lee; Joseph McCarville; Weixing Hu; Gurjit Kaur; Guoren Deng

Publisher
John Wiley and Sons
Year
1997
Tongue
French
Weight
75 KB
Volume
72
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

โœฆ Synopsis

In order to investigate the genomic instability associated with prostate cancer, 36 microsatellite marker loci on chro- mosomes 1p, 3p, 5q, 8p, 8q, 9p, 11q and13q were analyzed using microdissected samples from prostate cancer and adjoining microscopically normal tissues from the same slide. DNA was extracted from the normal and tumor cells of 40 microdissected prostate-cancer samples, amplified by PCR, and analyzed for microsatellite instability (MSI) using 36 different polymorphic DNA markers. In the present study, we have utilized a highly refined technique of PCR product separation on a sequencing gel, developed in our laboratory, which clearly shows high-quality results for the microsatellite instability in prostate cancer. The results of this study suggest that 45% (18 out of 40) showed genomic instability at a minimum of 1 locus; 4 cases each showed MSI at one and 2 loci, 4 cases had MSI at 3 loci, 3 cases showed MSI at 5 loci, while one case each showed MSI at 7, 8 and 15 loci. There was no significant correlation between the MSI and stage or grade of the tumors. This extensive study on genomic instability in prostate cancer found the occurrence of MSI to be very high, which suggests a role of MSI in the pathophysiology of prostate cancer.

๐Ÿ“œ SIMILAR VOLUMES

Expression of antioxidant enzymes in hum
Expression of antioxidant enzymes in human prostatic adenocarcinoma
โœ Baker, Andrew M.; Oberley, Larry W.; Cohen, Michael B. ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 157 KB ๐Ÿ‘ 2 views

## Background: Antioxidant enzymes (aes), which catalyze the conversion of reactive oxygen species (ros) to water, include catalase (cat), manganese-containing superoxide dismutase (mnsod), and copper and zinc-containing superoxide dismutase (cuznsod). previous work has indicated that mnsod, cat, a

Instability of chromosome 6 microsatelli
Instability of chromosome 6 microsatellite repeats in human cervical tumors carrying papillomavirus sequences
โœ Fjodor Kisseljov; Ljudmila Semionova; Ella Samoylova; Natalja Mazurenko; Elena K ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› John Wiley and Sons ๐ŸŒ French โš– 425 KB ๐Ÿ‘ 1 views

Loss of heterozygosity (LOH) in chromosome 6 in human squamous cervical carcinomas was analyzed in the long and short arms of the chromosome using 3 pairs of primers each. In all cases, normal adjacent tissue was used as control. Among 51 cases analyzed, we identified LOH or microsatellite instabili

Microsatellite instability during the im
Microsatellite instability during the immortalization and transformation of human breast epithelial cells in vitro
โœ Yajue Huang; Betsy Bove; Yuli Wu; Irma H. Russo; Xiaoqi Yang; Abdel Zekri; Jose ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 262 KB ๐Ÿ‘ 1 views

The objective of this study was to determine whether microsatellite instability (MSI) and loss of heterozygosity (LOH) are involved in the immortalization of human breast epithelial cells (HBECs) in vitro and in the early stages of their transformation by benzo[a]pyrene (BP) and 7,12-dimethylbenz[a]

The frequency of p53, k-ras mutations, a
The frequency of p53, k-ras mutations, and microsatellite instability differs in uterine endometrioid and serous carcinoma : Evidence of distinct molecular genetic pathways
โœ Sigurd F. Lax; Brian Kendall; Hironori Tashiro; Robbert J. C. Slebos; Lora Hedri ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 398 KB ๐Ÿ‘ 2 views

## BACKGROUND. The two most common types of uterine endometrial carcinoma, endometrioid (UEC) and serous (USC), differ in their histopathologic appearance and biologic behavior. Recent studies suggest that these differences may be associated with distinct molecular genetic alterations. ## METHODS.