Instability of chromosome 6 microsatellite repeats in human cervical tumors carrying papillomavirus sequences
✍ Scribed by Fjodor Kisseljov; Ljudmila Semionova; Ella Samoylova; Natalja Mazurenko; Elena Komissarova; Vera Zourbitskaya; Tatjana Gritzko; Vladimir Kozachenko; Michail Netchushkin; Semjon Petrov; Andrej Smirnov; Angel Alonso
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- French
- Weight
- 425 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Loss of heterozygosity (LOH) in chromosome 6 in human squamous cervical carcinomas was analyzed in the long and short arms of the chromosome using 3 pairs of primers each. In all cases, normal adjacent tissue was used as control. Among 51 cases analyzed, we identified LOH or microsatellite instability in 23% using primer D6S291 (located at position 6p21.3) and in 11% using primers D6S308 (6q16.3-6q27) and D6S270 (6q22.3-6q23.2). On the contrary, no significant LOH or genomic instabilities were detected with primers D6S306 (6p22.3-6p21.2), D6S299 (6p22.3-6p21.3) and D6S287 (6q21-6q23.3). Our results thus suggest the existence of instable loci at 3 regions of chromosome 6. Whether these loci contain putative tumor-suppressor genes or genes involved in cell cycle control remains unknown.