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High frequency embryogenesis and plant regeneration in isolated microspore culture of Brassica oleracea L.

✍ Scribed by Yoshihito Takahata; Wilfred A. Keller

Book ID
119136651
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
638 KB
Volume
74
Category
Article
ISSN
0168-9452

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Isolated microspores of various populations of three varieties of the Chinese cabbage pakchoi (Brassica rapa ssp. chinensis) were cultivated in vitro on NLN82 medium (Lichter 1982) and embryos and plantlets obtained with nine cultivars. The best embryo yield per bud was 57.4. A 33Β°C one day heat tre