Embryogenesis and plant regeneration of pakchoi (Brassica rapaL.ssp. chinensis)viain vitroisolated microspore culture

✍ Scribed by Ming Qing Cao; Yan Li; Fan Liu; Claire Doré

• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 636 KB
• Volume: 13
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00231964

✦ Synopsis

Isolated microspores of various populations of three varieties of the Chinese cabbage pakchoi (Brassica rapa ssp. chinensis) were cultivated in vitro on NLN82 medium (Lichter 1982) and embryos and plantlets obtained with nine cultivars. The best embryo yield per bud was 57.4. A 33°C one day heat treatment was generally necessary to induce embryogenesis. Analysis of ploidy level through flow cytometry for two cultivars indicated that haploids were present.