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High expression of Bcl-x protein in the developing human cerebellar cortex

✍ Scribed by O. Sohma; M. Mizuguchi; S. Takashima; M. Yamada; K. Ikeda; S. Ohta

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 917 KB
Volume: 43
Category: Article
ISSN: 0360-4012
DOI: 10.1002/(sici)1097-4547(19960115)43:2<175::aid-jnr5>3.0.co;2-d

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✦ Synopsis

Nakahara, Kawasaki (S.O.'), Japan

The gene bcl-x, which is related to a bcl-2, regulates programmed cell death. bcl-x may function in the development of the nervous system. We raised a polyclonal antibody against human Bcl-x protein, and investigated its distribution in the developing human cerebellum. Western blotting revealed that Bcl-x expression in the cerebellum is higher in the fetal, than in the postnatal period. Immunohistochemical studies of fetal brains localized intense Bcl-x immunoreactivity in the granule cell processes at 13-22 gestational weeks and in the Purkinje cell bodies at 24-38 weeks. The immunoreactivity decreased after birth, but was retained in the Purkinje cells at a low level until adulthood. These results suggested that Bcl-x expression in the cerebellum is developmentally regulated and involved specifically in the development of neuronal subpopulations.

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