High efficiency transformation of Escherichia coli with plasmids
β Scribed by Inoue, Hiroaki; Nojima, Hiroshi; Okayama, Hiroto
- Book ID
- 120060651
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 593 KB
- Volume
- 96
- Category
- Article
- ISSN
- 0378-1119
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π SIMILAR VOLUMES
UV irradiation of competent cells of Escherichia coli K12 produced an increase in the efficiency of transformation with plasmid DNA. This phenomenon has been called IPTE (increase in plasmid transformation efficiency) and is dependent on the activated state of the RecA protein. IPTE is independent o
The objectives of this **Second Edition** of **Biotechnology: A Laboratory Course** remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins