Cell volume changes correlate with efficiency of transformation of Escherichia coli with plasmid DNA
β Scribed by J.R. Saunders; A. Ball; Moira G.M. Brown; G.O. Humphreys; C. Edwards
- Book ID
- 109313698
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 284 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0378-1097
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Sheared DNA from RPI, and R plasmid from a strain of Pseudomonas aeruginosa, was used to transform other strains of P. aeruginosa and Escherichia coli. From transformed cells other plasmids like RPI were isolated. These deletion plasmids were conjugally transferrable and confer resistance mainly aga
## Abstract Plasmid DNA (pDNA) is an emerging experimental vaccine, produced in __E. coli__, initially targeted for viral diseases. Unlike traditional protein vaccines whose average dose is micrograms, the average dose of pDNA is on the scale of milligrams. Production yields are, therefore, importa