High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12
โ Scribed by Oeschger, Max P. ;Wiprud, Grant T.
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 637 KB
- Volume
- 178
- Category
- Article
- ISSN
- 0026-8925
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In studies on thymineless death in Escherichia coli K12, it was noted that certain thymine requiring mutants were inhibited by thymidine. The pattern of inhibition varied with the conditions and media employed. Accumulation of deoxyribose-5-phosphate as a possible reason for inhibition is ruled out
Temperature-sensitive (ts) mutations were isolated within a ribosomal protein gene (rpsL) of Escherichia coli K12. Mutations were mapped by complementation using various transducing phages and plasmids carrying the rpsL gene, having either a normal or a defective promoter for the rpsL operon. One of