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High-Efficiency RNA Interference in Human Embryonic Stem Cells

โœ Scribed by Holm Zaehres; M. William Lensch; Laurence Daheron; Sheila A. Stewart; Joseph Itskovitz-Eldor; George Q. Daley

Book ID
111843466
Publisher
AlphaMed Press
Year
2005
Tongue
English
Weight
232 KB
Volume
23
Category
Article
ISSN
1066-5099

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## Abstract Summary: We examined whether suppression of Oct4 via RNA interference (RNAi) would affect embryonic stem (ES) cell lineage choice. Cells were transfected with plasmids containing an independently expressed reporter gene and an RNA polymerase type III promoter to constitutively express s