Amylase and trypsin inhibitors are proteins which inhibit digestive enzymes. The loss of the activity of these enzymes produces a reduction of starch and protein digestion. Amylase and trypsin inhibitor activity have been investigated in seeds of 20 cowpea lines in trying to establish their role in
High antinutrient levels and bruchid resistance of cowpea (Vigna unguiculata) seeds
โ Scribed by A. R. Piergiovanni; C. Della Gatta; L. Sergio; P. Perrino
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 293 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0014-2336
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โฆ Synopsis
Seeds of some cowpea varieties, characterized by different degrees of storage pest resistance, were analyzed for inhibitors of the following enzymes: porcine amylase, Bacillus amylase, bovine chymotrypsin and trypsin. A broad variation was observed among samples for all tested inhibitors. Submitting the experimental data to the principal component analysis it has been observed that resistant lines are characterized by high levels of both antitryptic and antiamylasic activity. Moreover a high activity of a single inhibitor class is typical of the bruchid susceptible lines. Hence, breeding for high contents of these protein inhibitors could be an effective way of obtaining lines that are naturally resistant to storage pest attack. The limit of this breeding strategy seems to be the difficulty to reduce the antiamylasic activity before eating.
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Damage by insects and mould, proximate composition, cooking rate and functional properties of sun-dried and stored cowpea (Vigna unguiculata) seeds were evaluated. Cowpea samples spread between black polythene ยฎlms were placed on cement (CS), Wooden (WS) and corrugated iron sheet (CIS) surfaces, res