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High Affinity, Sequence Specific DNA Binding by Synthetic Tripyrrole–Peptide Conjugates

✍ Scribed by Juan B. Blanco; Olalla Vázquez; José Martínez-Costas; Luis Castedo; José L. Mascareñas

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
417 KB
Volume
11
Category
Article
ISSN
0947-6539

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✍ S. Pierrou; S. Enerback; P. Carlsson 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 695 KB

We describe a rapid and sensitive method to isolate sets of high-affinity binding sites for sequence-specific DNA binding proteins. The DNA binding domain of the protein is expressed in Escherichia coli as a fusion with glutathione S-transferase (GST). Binding reactions are set up with total soluble