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High-affinity cytochalasin b binding to normal and transformed BALB/3T3 cells

✍ Scribed by Susan J. Atlas; Shin Lin

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
431 KB
Volume
89
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

To study the molecular basis of changes in sugar uptake rate in cultured mouse fibroblasts with different physiological states, we have measured the high affinity binding of [^3^H] cytochalasin B, a potent sugar transport inhibitor, to actively growing and contact inhibited Balb/3T3 cells as well as to 3T12 and SV3T3 cells. Binding was the same whether the cells were detached from dishes with EDTA or trypsin. The amount of drug bound to intact cells measured with a centrifugation assay was essentially the same as that bound to cell sonicates measured with equilibrium dialysis. Cytochalasin B binding to intact cells was extremely rapid and reversible over a wide range of drug concentrations, and was not affected by 0.1 M D‐glucose or L‐glucose in the assay medium. Actively growing and contact inhibited 3T3 cells had a similar number of high affinity cytochalasin B binding sites per cell, while 3T12 and SV3T3 cells had one third to one fourth the number of sites per cell. However, the number of sites per μg cellular protein appeared to be similar for cells in all of the physiological states examined.

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