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Hexosaminidase a deficient adults: Presence of α chain precursor in cultured skin fibroblasts

✍ Scribed by Amos Frisch; Dana Baram; Ruth Navon


Book ID
115758129
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
651 KB
Volume
119
Category
Article
ISSN
0006-291X

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✍ S. Fujibayashi; R. Minami; Y. Ishikawa; K. Wagatsuma; T. Nakao; S. Tsugawa 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 324 KB

On DEAE cellulose column chromatography, alpha-L-iduronidase in cultured skin fibroblasts was resolved into two distinct components, forms A and B. They had similar Km values for 4-methylumbelliferyl-alpha-L-iduronide, but differed in pH optima and thermal stability. Form B was more heat-stable than