✦ LIBER ✦

Heterotrimeric configuration is essential to the adhesive function of laminin

✍ Scribed by J. C. Lissitzky; P. Cantau; P. M. Martin

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 753 KB
Volume: 48
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.240480206

No coin nor oath required. For personal study only.

✦ Synopsis

Mouse PFHR9 larninin, B1 B2-heterodimers, and free B1-chains were separated from one another by gel filtration on Superose 6. The cell attachment promoting activity of these species was measured after imrnunoprecipitation with monoclonal anti-larninin antibodies coupled to Sepharose 6MB beads. These antibodies, which did not react with the laminin E 8 fragment, were directed against epitopes in the NH2-terminus of the laminin B1 -chain and in the central region of larninin. After incubation with purified EHS larninin, the irnmunosorbents revealed efficient adhesion substrates for a rat rhabdomyosarcoma cell line which attached preferentially to the larninin E8 fragment. Although both were irnrnunoprecipitated efficiently, B1 B2-heterodimers and B1 -chains, unlike PFHR9 larninin, did not support the attachment of RMS cells. On a molar basis B1 B2-heterodimers were 24 times less efficient than PFHR9 laminin or EHS larninin in supporting cell attachment. These data suggest that heterotrirneric configuration is essential to the adhesive function of the laminin E8 fragment.

📜 SIMILAR VOLUMES

T lymphocyte adhesion to the fibronectin

T lymphocyte adhesion to the fibronectin and laminin components of the extracellular matrix is regulated by the CD4 molecule

✍ Rami Hershkoviz; Shmuel Miron; Iran R. Cohen; Ariel Miller; Ofer Lider 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 765 KB

Expression of lysyl oxidase from cDNA co

Expression of lysyl oxidase from cDNA constructs in mammalian cells: The propeptide region is not essential to the folding and secretion of the functional enzyme

✍ Herbert M. Kagan; Vemuri B. Reddy; Maria V. Panchenko; Narasimhan Nagan; Andra M 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 912 KB

## Abstract Rat aortic lysyl oxidase cDNA was expressed under a metallothionein promoter in Chinese hamster ovary cells using a dihydrofolate reductase selection marker. One methotrexate‐resistant cell line, LOD‐06, generated by transfecting with full‐length cDNA, yielded lysyl oxidase proteins con

ChemInform Abstract: Molecular Biologica

ChemInform Abstract: Molecular Biological Approach to Functions of Telencephalin, a Cell Adhesion Molecule and HNK-1 Carbohydrate Epitope, which Is Commonly Expressed on Cell Adhesion Molecules in the Nervous System

✍ Shogo Oka 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 25 KB 👁 2 views

## Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable v

BTN1, a Yeast Gene Corresponding to the

BTN1, a Yeast Gene Corresponding to the Human Gene Responsible for Batten's Disease, is Not Essential for Viability, Mitochondrial Function, or Degradation of Mitochondrial ATP Synthase

✍ PEARCE, DAVID A.; SHERMAN, FRED 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 142 KB 👁 2 views

The Saccharomyces cerevisiae gene BTN1, encodes a 408 amino acid putative integral membrane protein, which is 39% identical and 59% similar to the human Cln3p, whose mutant forms are responsible for Batten's disease and for a diminished degradation of mitochondrial ATPase synthase subunit c. Disrupt