Heterogeneity in Deoxyribonucleic Acids: II. Dependence of the Density of Deoxyribonucleic Acids on Guanine–Cytosine Content
✍ Scribed by SUEOKA, NOBORU; MARMUR, JULIUS; DOTY, PAUL
- Book ID
- 109601254
- Publisher
- Nature Publishing Group
- Year
- 1959
- Tongue
- English
- Weight
- 352 KB
- Volume
- 183
- Category
- Article
- ISSN
- 0028-0836
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## Abstract An explicit relation between the percentage (__G__ + __C__)‐content __X__~__GC__~, and the melting temperature (__T__~__m__~) of a natural DNA, is derived froma a statistical mechanics calculation with due consideration given to the base pair bonding and stacking interactions. The well‐
A rapid high performance liquid chromatographic (HPLC) method is described which offers a fast quantitative procedure for determining the percentage of guanine (G) plus cytosine (C) in DNA preparations after acid hydrolysis in 88% formic acid. As little as 10 Fg of DNA is amenable to this analysis.