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Herpes Simplex Virus as a Source of Thymidine Kinase for Thymidine Kinase-Deficient Mouse Cells: Suppression and Reactivation of the Viral Enzyme

✍ Scribed by Richard L. Davidson, S. J. Adelstein and Michael N. Oxman


Book ID
123651663
Publisher
National Academy of Sciences
Year
1973
Tongue
English
Weight
787 KB
Volume
70
Category
Article
ISSN
0027-8424

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The herpes simplex type 1 biochemically transformed human cell line, HB-1, was fused with thymidine kinase deficient rodent cells, and 18 hybrids were isolated using the HAT-ouabain selection system. The selected enzyme, viral thymidine kinase, was present in all 18 hybrids. In 16 of 18 hybrids the