Hepatitis C Assays: The Pitfalls of Polymerase Chain Reaction and Genotyping
β Scribed by R. Todd Frederick
- Book ID
- 107540518
- Publisher
- Current Science Inc.
- Year
- 2010
- Tongue
- English
- Weight
- 119 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1540-3416
No coin nor oath required. For personal study only.
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## Abstract A polymerase chain reaction (PCR)βbased assay using primers against the hepatitis C core gene has been described [Okamoto et al. (1992a): __Journal of General Virology__ 73:673β679]. Within the two major HCV genotypes 1 and 2, the Okamoto system identifies two subtypes each (1a,1 b and
The newly developed immunoblot assay, RlBA SIA (recombinant and synthetic polypeptide immunoblot assay), Chiron, Calif., was compared with the commercially available second generation recombinant immunoblot assay (RIBA-2) for the detection of antibody to hepatitis C virus (anti-HCV). The two immunob
## Abstract Hepatitis C virus (HCV) exhibits considerable sequence variability and circulates in the blood at extremely low levels. Current methods for detecting HCV RNA are based mostly on nested polymerase chain reaction (PCR), in which part of the first amplification product is reamplified in th