Hepatic intracellular pH in Vivo using F-quene 1 and 19F NMR spectroscopy

✍ Scribed by John S. Beech; Richard A. Iles

• Book ID: 102530684
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 364 KB
• Volume: 19
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.1910190229

✦ Synopsis

Abstract

Intracellular pH (pHi) has been determined in vivo in livers from anaesthetized rats using both ^31^P NMR and ^19^F NMR spectroscopy. In the ^31^P NMR study pH, determined from the chemical shift of endogenous P~i~, was found to be 7.26 ± 0.02. In the ^19^F NMR study on a separate group of animals pH~i~ was determined from the chemical shift of F‐Quene 1 infused via the portal vein and found to be 7.18 ± 0.01. In approximately half of the ^19^F NMR studies no ^19^F signal could be detected in the liver probably because of loss of F‐Quene 1 in the circulation. The small discrepancy between pH~i~ determined by the two probes could be caused by different intra‐ or intercellular distributions or by unknown effects of F‐Quene 1 on metabolism. © 1991 Academic Press, Inc.