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Hemolysis-in-gel test in immunity surveys and diagnosis of rubella

✍ Scribed by Pertti Väänänen; Antti Vaheri

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
411 KB
Volume
3
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

The hemolysis‐in‐gel (HIG) technique was adapted for rubella antibody determinations. Use of sucrose gradient purified virus and its coupling with CrCl~3~ to chicken erythrocytes resulted in gel plates that could be stored for several weeks and were suitable for reproducible antibody determinations. In a serological survey of young healthy adults the HIG values (range > 2–13 mm) were in close correlation to those obtained by the HI test (> 10 to 320). The HIG test seems well suited for screening the need of vaccination. Seronegative sera (HIG > 2, HI> 10) gave without heat inactivation hemolysis zones ranging from 4 to 6.5 mm. Although the present rubella HIG test did not measure IgM antibodies, the test, by virtue of its accuracy and sensitivity — extending to antibody levels corresponding to HI titers 2–10 — provides a simpler and more rapid means for diagnosis of rubella infections than the conventional HI and CF tests.

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