𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Evaluation of solid-phase enzyme-lmmunoassay procedure in immunity surveys and diagnosis of rubella

✍ Scribed by Antti Vaheri; Eeva-Marjatta Salonen

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
561 KB
Volume
5
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

In the development of optimized micro‐ and macromethods for solid‐phase rubella enzyme‐immunoassay (EIA), the effect of various technical parameters was studied. Special emphasis was placed on elimination of defective polystyrene material, purity and quantity of immobilized antigen, use of reference sera in each microplate or macroprocedure, use of nonionic detergent in washing and diluent buffers, and use of a colourless enzyme substrate. A single serum dilution, analysed in duplicate, was sufficient provided the procedure was standardized. In quantitation of serum rubella antibodies, EIA gave values correlating well with those obtained with the single radial haemolysis test but not with low haemagglutination inhibition titres. The EIA procedure proved to be a useful and reliable serological tool in immunity surveys. When complemented by a rubella IgM test, EIA could also be used in diagnosis of recent rubella infections as a convenient primary serological test.

📜 SIMILAR VOLUMES

Hemolysis-in-gel test in immunity survey
Hemolysis-in-gel test in immunity surveys and diagnosis of rubella
✍ Pertti Väänänen; Antti Vaheri 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 English ⚖ 411 KB

## Abstract The hemolysis‐in‐gel (HIG) technique was adapted for rubella antibody determinations. Use of sucrose gradient purified virus and its coupling with CrCl~3~ to chicken erythrocytes resulted in gel plates that could be stored for several weeks and were suitable for reproducible antibody de

Evaluation of a solid phase extraction p
Evaluation of a solid phase extraction procedure for the determination of pesticide residues in foodstuffs
✍ Barwick, Vicki J; Ellison, Stephen L?R; Lacey, Sharon J; Mussell, Chris R; Lucki 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 89 KB 👁 2 views

An evaluation of a method based on solid phase extraction (SPE) as a clean-up stage for the determination of pesticide residues in composite vegetable and cabbage samples is presented. Samples are extracted with acetonitrile and the extract is loaded onto an SPE cartridge. The pesticides are eluted

Rapid formation of the immune complexes
Rapid formation of the immune complexes on solid phase in the immune complex transfer enzyme immunoassay for HIV-1 p24 antigen and antibody IgGs to HIV-1
✍ Setsuko Ishikawa; Seiichi Hashida; Kazuya Hashinaka; Akio Adachi; Shinichi Oka; 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 89 KB 👁 2 views

In order to perform the immune complex transfer enzyme immunoassays for HIV-1 p24 antigen and antibody IgGs to HIV-1 p17, reverse transcriptase and gp41 antigens as rapidly as possible, methods for rapid formation of the immune complexes on solid phase are described. HIV-1 p24 antigen was reacted wi