Hemoglobin and ferritin synthesis in erythroid cells in prolonged marrow cell cultures

✍ Scribed by Thomas G. Gabuzda; Ruth K. Silver; Mary Melvin; Constance Windus; Carl Sachs

• Publisher: John Wiley and Sons
• Year: 1969
• Tongue: English
• Weight: 630 KB
• Volume: 74
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040740307

✦ Synopsis

A method is described which maintains viable erythroid cells in tissue culture for periods from nine to twenty days. These cells appear predominantly as small round cells with scanty cytopIasm. They synthesize both heme and globin and are relatively more numerous free in suspension than in the adherent monolayer. Ferritin isomorph may serve as a convenient marker in tissue culture of cells of erythroid origin, suggesting that such cells may persist despite a completely transformed appearance and a loss of the ability to produce hemoglobin.