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Helix–coil transition in nucleoprotein—theory and applications

✍ Scribed by Hsueh Jei Li

Book ID
102760269
Publisher
Wiley (John Wiley & Sons)
Year
1973
Tongue
English
Weight
521 KB
Volume
12
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

A general theory of helix–coil transition of irreversibly complexed nucleoproteins is presented. The equations are tested by experimental results in basic polypeptide–DNA complexes, nucleohistone I and pea bud nucleohistones. They show good agreement between theory and experiments. The theory provides direct measurement of a fraction of DNA base pairs covered by proteins, yielding a value of about 75% histone‐covered base pairs in pea bud nucleohistone. It also provides a measurement of an average number of amino acid residues per nucleotide in protein‐bound regions. This number varies from 1.0 to 1.4 in DNA–polylysine or DNA–polyarginine and from 2.9 to 3.3 in nucleohistone Ia, Ib, f1, and pea bud nucleohistone.

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