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Helix → β conformational transition of poly(L-lysine) on dye binding

✍ Scribed by P. V. Scaria; M. Atreyi; M. V. R. Rao

Publisher
Wiley (John Wiley & Sons)
Year
1986
Tongue
English
Weight
490 KB
Volume
25
Category
Article
ISSN
0006-3525

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✦ Synopsis

Binding of an azo dye, 4'-dimethyl amino azo benzene-4-carboxylic acid (DAAC) to poly(L-lysine) (PLL) in basic aqueous solutions at 20°C has been studied. The azo dye was found to bind to PLL when its side-chain amino groups are in the uncharged state. This was found to be a cooperative phenomenon, and the binding constant and cooperativity factor have been evaluated. The binding of the dye was found to result in a conformational transition of PLL from the &-helix to the p-sheet, which in turn helps in increased dye binding.

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