## Abstract Heptafluorobutyl chloroformate (HFBCF), a recently introduced derivatization reagent, was examined in enantioseparation of amino acids (AAs) by GC. Twenty proteinogenic AAs, plus ornithine, cystine and 4‐fluorophenylalanine (internal standard) were treated with the reagent and separatio
Heliflex chirasil-Val; GC of amino acid enantiomers
✍ Scribed by Abe, I. ;Kuramoto, S. ;Musha, S.
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 405 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0935-6304
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📜 SIMILAR VOLUMES
decomposition was observed. The reaction completeness was checked with mobile phase B because in mobile phase A the promethazine peak, R t 3.10 min, did not separate from other peaks. A baseline separation was obtained for the diastereoisomers with mobile phase A (Figure 3) with a resolution of 2.0.
Several dipeptides have been separated into stereoisomers (enantiomers, diastereoisomers, sequential isomers) by GC on the chiral stationary phase Chirasil-Val. Sufficiently volatile N-TFA-dipeptide methyl esters have been formed by derivatization at ambient temperature, thus avoiding racemization (
## Abstract An enantiomeric mixture of amino acids is derivatized to give the respective __N(O)__‐perfluoroacyl alkyl esters which can be resolved directly by gas chromatography on a Chirasil‐Val capillary column. The retention times, resolution numbers, and elution order are reported.