Heavily methylated amplified DNA in transformants of Neurospora crassa
โ Scribed by Bull, John H.; Wootton, John C.
- Book ID
- 109736332
- Publisher
- Nature Publishing Group
- Year
- 1984
- Tongue
- English
- Weight
- 508 KB
- Volume
- 310
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/310701a0
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Repeated DNA sequences of Neurospora crassa were isolated and characterized. Approximately 10-12% of N. crassa DNA sequence were repeated, of which 7.3% were found to be transcribed in mid-log phase of mycelial growth as measured by DNA:RNA hybridization. It is suggested that part of repetitive DNA
Transcriptional properties of an "albino" mutant, a "slime" mutant and a wild type strain of Neurospora crassa have been compared by quantitative estimates of DNA:RNA hybridization. The RNAs isolated from these strains were incubated with 32P-DNA of the wild type strain. The DNA:RNA hybrids were iso