Heat Shock Protein 60kDa in breast cancer tissue and cell lines
β Scribed by H. Park; S. Park; M. Choi; M. Hur; S. Han
- Book ID
- 119597943
- Publisher
- Elsevier Science
- Year
- 2008
- Tongue
- English
- Weight
- 60 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1359-6349
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## Abstract To investigate whether the 60βkDa heat shock chaperonin protein (hsp60) is present on the surface of mammalian cells, we used immunogold labeling of intact cells and backscattered electron imaging to image gold particles. Chinese hamster ovary cells and the human leukemic CD4βpositive T
Seven different cell lines were analyzed by flow cytometry to d u a t e the variation i n heat shock protein hsp70 through the cell cycle. Inducible (hsp72) or both constitutive and inducible (hsp70) heat shock proteins were measured with monoclonal antibodies, and the cell cycle distribution was si