Exposure of 9L rat brain tumor cells to 40-100 Β΅M CdCl 2 for 2 h leads to an induction of a wide spectrum of heat shock proteins (HSPs). We have demonstrated that induction of the 70-kDa HSP (HSP70) and enhanced expression of its cognate (HSC70) by cadmium are concentration dependent and that the in
Levels of 70-kDa heat shock protein through the cell cycle in several mammalian cell lines
β Scribed by Haiying Hang; Michael H. Fox
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 482 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0196-4763
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β¦ Synopsis
Seven different cell lines were analyzed by flow cytometry to d u a t e the variation i n heat shock protein hsp70 through the cell cycle. Inducible (hsp72) or both constitutive and inducible (hsp70) heat shock proteins were measured with monoclonal antibodies, and the cell cycle distribution was simultaneously measured with propidium iodide. Cell lines analyzed were Rat-1, HR-24 (Rat-1
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