Heat-Activation Kinetics of Endospores of Bacillus subtilis
β Scribed by F. F. BUSTA; Z. JOHN ORDAL
- Book ID
- 108797043
- Publisher
- Institute of Food Technologists
- Year
- 1964
- Tongue
- English
- Weight
- 814 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0022-1147
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Recombinant plasmid pCED3 is structurally unstable in Bacillus subtilis cultures. We have previously shown that stability can be independently increased by changing from a complex medium supporting high growth rates to a chemically-defined medium supporting a lower growth rate and removal of a 4.77-
Bacillus subtilis spores were suspended in 0.1% NaCl solution (ca. 10 7 CFU/mL) and treated by conventional or ohmic heating under identical temperature histories. Temperatures tested were in the range of 88 to 99Β°C. Survival curves and calculated D values showed significantly higher lethality for s