V. 1: -- Part I. Rna Synthesis -- I.1. Enzymatic Rna Synthesis Using Bacteriophage T7 Rna Polymerase: / Heike Gruegelsiepe [and Others] -- Production Of Rnas With Homogeneous 5' And 3' Ends / Mario Mörl [and Others] -- Rna Ligation Using T4 Dna Ligase / Mikko J. Frilander, Janne J. Turunen -- T4 Rna Ligase / Tina Persson [and Others] -- Co- And Post-transcriptional Incorporation Of Specific Modifications Including Photoreactive Groups Into Rna Molecules / Nathan H. Zahler, Michael E. Harris -- 3'-terminal Attachment Of Fluorescent Dyes And Biotin / Dagmar K. Willkomm, Roland K. Hartmann -- I.2. Chemical Rna Synthesis: -- Chemical Rna Synthesis, Purification And Analysis / Brian S. Sproat -- Modified Rnas As Tools In Rna Biochemistry / Thomas E. Edwards, Snorri Th. Sigurdsson -- Part Ii. Structure Determination -- Ii. 1. Molecular Biology Methods: -- Direct Determination Of Rna Sequence And Modification By Radiolabeling Methods / Olaf Gimple, Astrid Schön -- Probing Rna Structures With Enzymes And Chemcials In Vitro And In Vivo / Eric Huntzinger [and Others] -- Study Of Rna-protein Interactions And Rna Structure In Ribonucleoprotein Particles / Virginie Marchand [and Others] -- Terbium(iii) Footprinting As A Probe Of Rna Structure And Metal-binding Sites / Dinari A. Harris, Nils G. Walter -- Pb²-induced Cleavage Of Rna / Leif A. Kirsebom, Jerzy Ciesiolka -- In Vivo Determination Of Rna Structure By Dimethylsulfate / Christina Waldsich, Renée Schroeder -- Probing Structure And Binding Sties On Rna By Fenton Cleavage / Gesine Bauer, Christian Berens -- Measuring The Stoichiometry Of Magnesium Ions Bound To Rna / A.j. Andrews, Carol Fierke -- Nucleotide Analog Interference Mapping And Suppression: Specific Applications In Studies Of Rna Tertiary Structure, Dynamic Helicase Mechanism And Rna-protein Interactions / Olga Fedorova [and Others] -- Nucleotide Analog Interference Mapping: Application To The Rnase P System / Simona Cuzic, Roland K. Hartmann -- Identification And Characterization Of Metal Ion Binding By Thiophilic Metal Ion Rescue / Eric L. Christian -- Identification Of Divalent Metal Ion Binding Sites In Rna/dna-metabolizing Enzymes By Fe(ii)-mediated Hydroxyl Radical Cleavage / Yan-guo Ren [and Others] -- Protein-rna Crosslinking In Native Ribonucleoprotein Particles / Henning Urlaub [and Others] -- Probing Rna Structure By Photoaffinity Crosslinking With 4-thiouridine And 6-thioguanosine / Michael E. Harris, Eric L. Christian -- Ii. 2. Biophysical Methods: -- Structural Analysis Of Rna And Rna-protein Complexes By Small-angle X-ray Scattering / Tao Pan, Tobin R. Sosnick -- Temperature-gradient Gel Electrophoresis Of Rna / Detlev Riesner, Gerhard Steger -- Uv Melting, Native Gels And Rna Conformation / Andreas Werner -- Sedimentation Analysis Of Ribonucleoprotein Complexes / Jan Medenbach [and Others] -- Preparation And Handling Of Rna Crystals / Boris François [and Others] -- Ii. 3. Fluorescence And Single Molecule Studies: -- Fluorescence Labeling Of Rna For Single Molecule Studies / Filipp Oesterhelt [and Others] -- Scanning Force Microscopy And Scanning Force Spectroscopy Of Rna / Wolfgang Nellen -- V. 2. -- Comparative Analysis Of Rna Secondary Structure: 6s Rna / James W. Brown, J. Christopher Ellis -- Secondary Structure Prediction / Gerhard Steger -- Modeling The Architecture Of Structures Rnas Within A Modular And Hierarchical Framework / Benoît Masquida, Eric Westhof -- Modeling Large Rna Assemblies Using A Reduced Representation / Jason A. Mears [and Others] -- Molecular Dynamics Simulations Of Rna Systems / Pascal Auffinger, Andrea C. Vaiana -- Seeking Rna Motifs In Genomic Sequences / Matthieu Legendre, Daniel Gautheret -- Approaches To Identify Novel Non-messenger Rnas In Bacteria And To Investigate Their Biological Functions: Rna Mining / Jörg Vogel, E. Gerhart H. Wagner -- Approaches To Identify Novel Non-messenger Rnas In Bacteria And To Investigate Their Biological Functions: Functional Analysis Of Identified Non-mrnas / E. Gerhart H. Wagner, Jörg Vogel -- Experimental Rnomics: A Global Approach To Identify Non-coding Rnas In Model Organisms / Alexander Hüttenhofer -- Large-scale Analysis Of Mrna Splice Variants By Microarray / Young-soo Kwon [and Others] -- Part Iv. Analysis Of Rna Function -- Iv. 1 Rna-protein Interactions In Vitro -- Use Of Rna Affinity Matrices For The Isolation Of Rna-binding Proteins / Steffen Schiffer [and Others] -- Biotin-based Affinity Purification Of Rna-protein Complexes / Zsofia Palfi [and Others] -- Immunoaffinity Purification Of Spliceosomal And Small Nuclear Ribonucleoprotein Complexes / Cindy L. Will [and Others] -- Northwestern Techniques For The Identification Of Rna-binding Proteins From Cdna Expression Libraries And The Analysis Of Rna-protein Interactions / Ángel Emilio Martínez De Alba [and Others] -- Iv. 2. Rna-protein Interations In Vivo: -- Fluorescent Detection Of Nascent Transcripts And Rna-binding Proteins In Cell Nuclei / Jennifer A. Geiger, Karla M. Neugebauer -- Identification And Characterization Of Rna-binding Proteins Through Three-hybrid Analysis / Felicia Scott, David R. Engelke -- Analysis Of Alternative Splicing In Vivo Using Using Minigenes / Yesheng Tang [and Others] -- Iv. 3 Selex: -- Artificial Selection: Finding Function Amongst Randomized Sequences / Ico De Zwart [and Others] -- Aptamer Selection Against Biological Macromolecules: Proteins And Carbohydrates / C. Stefan Vörtler, Maria Milovinkova -- In Vivo Selex Strategies / Thomas A. Cooper -- In Vitro Selection Against Small Targets / Dirk Eulberg [and Others] -- Selex Strategies To Identify Antisense And Protein Target Sites In Rna Or Heterogeneous Nuclear Ribonucleoprotein Complexes / Martin Lützelberger [and Others] -- Part V. Rnai -- Gene Silencing Methods For Mammalian Cells: Application Of Synthetic Short Interfering Rnas / Matthias John [and Others]. Edited By Roland K. Hartmann ... [et Al.]. Includes Bibliographical References And Index.