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Handbook of ELISPOT Volume 302 || Interferon-γ ELISPOT Assay for the Quantitative Measurement of Antigen-Specific Murine CD8+ T-Cells

✍ Scribed by Kalyuzhny, Alexander E.


Book ID
111868914
Publisher
Humana Press
Year
2005
Weight
128 KB
Category
Article

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Blood lymphocytes from HLA-A\*0201-subtyped melanoma patients and healthy controls were screened for the presence of T cells specific for HLA-A\*0201-binding melanoma and viral peptide antigens by the enzyme-linked immunoSPOT (ELISPOT) assay. CD8(+) cells were tested for peptide-specific IFN-gamma r