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The use of HLA-A*0201-transfected K562 as standard antigen-presenting cells for CD8+ T lymphocytes in IFN-γ ELISPOT assays

✍ Scribed by Cedrik M Britten; Ralf G Meyer; Tanja Kreer; Ingo Drexler; Thomas Wölfel; Wolfgang Herr


Book ID
114050890
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
320 KB
Volume
259
Category
Article
ISSN
0022-1759

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Blood lymphocytes from HLA-A\*0201-subtyped melanoma patients and healthy controls were screened for the presence of T cells specific for HLA-A\*0201-binding melanoma and viral peptide antigens by the enzyme-linked immunoSPOT (ELISPOT) assay. CD8(+) cells were tested for peptide-specific IFN-gamma r