Guidelines for experimental design and data analysis of proteomic mass spectrometry-based experiments
✍ Scribed by Karin Barnouin
- Publisher
- Springer
- Year
- 2010
- Tongue
- English
- Weight
- 88 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0939-4451
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Abstract The proteome, defined as an organism's proteins and their actions, is a highly complex end‐effector of molecular and cellular events. Differing amounts of proteins in a sample can be indicators of an individual's health status; thus, it is valuable to identify key proteins that serve as
## Abstract A novel class of chemically modified iodo‐containing resins with isotope‐labeled tagging for mass spectrometry‐based proteome analysis is described. This iodo‐containing resin contains a thiol‐reactive group that is used to capture the cysteine (Cys)‐containing peptides from peptide mix
Separation and relative quantitation of complex protein mixtures remain two of the most challenging aspects of proteomics. Here an advanced technique called fluorescence difference 2-D gel electrophoresis technology (2D-DIGE) has been applied to a model system study of the Escherichia coli proteome
Experimental design of (13)C-tracer studies for metabolic flux analysis with mass spectrometric determination of labeling patterns was performed for the central metabolism of Corynebacterium glutamicum comprising various flux scenarios. Ratio measurement of mass isotopomer pools of Corynebacterium p