A novel class of chemically modified iodo-containing resins: design, synthesis and application to mass spectrometry-based proteome analysis
✍ Scribed by Li Zhang; Yin-Long Guo; Han-Qing Liu
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 173 KB
- Volume
- 39
- Category
- Article
- ISSN
- 1076-5174
- DOI
- 10.1002/jms.615
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✦ Synopsis
Abstract
A novel class of chemically modified iodo‐containing resins with isotope‐labeled tagging for mass spectrometry‐based proteome analysis is described. This iodo‐containing resin contains a thiol‐reactive group that is used to capture the cysteine (Cys)‐containing peptides from peptide mixtures, one ‘tag’ amino acid, and an aminomethyl polystyrene resin with Rink Amide Linker. The ‘tag’ amino acid is synthesized in both heavy and light isotope‐coded forms and therefore permits the direct relative quantification of peptides/proteins through mass spectrometric analysis. In the iodo‐containing resin strategy, the Cys‐containing peptides of two samples covalently captured by either light or heavy iodo‐containing resin were mixed and washed extensively under stringent conditions. Then the Cys‐containing peptides were retrieved by acid‐catalyzed elution. Finally, the eluted peptides were directly analyzed by micro liquid chromatography/mass spectrometry for identification and relative quantification. The iodo‐containing resins were synthesized by a simple but effective method. Their abilities to identify and quantify the Cys‐containing part in two samples were proved by the analysis of mixtures of amino acids, peptides and proteins. Copyright © 2004 John Wiley & Sons, Ltd.